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Pfizer BioNTech Covid-19 Lipid Nanoparticle Components and Vaccine Administration

In 2020-2021, the lipid nanoparticles (LNPs) emphasized as the forefront of science and technology when Pfizer/BioNTech and Moderna vaccines used those formulations for delivering messenger RNA (mRNA) and protected the mRNA molecules from degradation. Highly encapsulated LNP-mRNA led to effectiveness with intracellular delivery and endosomal escape in the nucleoside-modified mRNA-LNP. Specifically the lipid nanoparticle in Pfizer - BioNTech vaccines included the following formulation components: Potassium chloride Monobasic potassium phosphate Sodium chloride Dibasic sodium phosphate dihydrate Sucrose Lipid 1: ((4-hydroxybutyl)azanediyl)bis(hexane-6,1-diyl)bis(2- hexyldecanoate), Lipid 2: 2-[(polyethylene glycol)-2000]-N,N-ditetradecylacetamide, 1,2-distearoyl-snglycero-3-phosphocholine, and cholesterol) The Pfizer-BioNTech COVID-19 Vaccine is stored in a freezing condition to protect the mRNA stability, and it is supplied as a frozen [between -80°C to -60°C (- 112°F to -76°F)] in five-dose vials....

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COVID-19 Vaccines and the Role of Lipid Nanoparticles Delivery

mRNA vaccines are using a promising technology and an alternative option to conventional vaccine approaches. Some of their benefits include their low-cost manufacture, high potency, safety, and rapid development. The mechanism by which they work in the human body is by triggering the immune system leading to the development of antibodies and cytotoxic T cells. However, some of their drawbacks included their instability and inefficient in vivo delivery of mRNA. The key to an effective mRNA vaccine is efficient in vivo delivery which is provided by lipid nanoparticles. They can facilitate endosomal escape. They can be targeted to the selected cell type, and they can be delivered with adjuvants. The era of COVID-19 has brought to the forefront mRNA-based vaccines. At present, lipid...

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Overview of Pharmaceutical Drug Discovery and Development

Established and defined by the US Food and Drug Administration (US-FDA), drug development includes the five stages below: Discovery and Development Preclinical Research Clinical Research FDA Review FDA Post-Market and Safety Monitoring  Discovery and Development: In this step, researchers in academic institutions, government laboratories, and pharmaceutical companies' Research and Development discover new pharmaceutical drugs and formulations. In this step, the pharmaceutical development purpose is to stop or reverse the effect of a specific disease or illness. In these steps, experiments are designed with various biomolecules to identify the benefits of a particular formulation that works for a disease or condition. Moreover, the unanticipated effect of those formulations is recognized for any adverse effects on patients. Lastly, the new technologies are incorporated...

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