ProFECT(mRNA) Plus: Translational Lipid Nanoparticles for mRNA Encapsulation and Delivery
ProFECT for Gene Therapy Delivery:
T&T Scientific’s ProFECT™(mRNA) Transfection Kit is a proprietary and patent-protected lipid-based transfection system designed for safe and effective nucleic acid delivery in a broad range of cell types with high viability and low lipid-associated toxicity. ProFECT™, a natural lipid composition comprised of non-cationic phospholipid and sterol components, is modified with functional amino acids for highly efficient encapsulation of mRNA and enhanced intracellular delivery. This easy-to-use lipid formulation is intended for preclinical in vitro studies aimed at translational development of mRNA therapeutics.
A major challenge in nanomedicine is the translation from laboratory formulation to large scale production and has limited clinical success in the field of nanotherapeutics. While translational success is limited with conventional drug delivery systems, the development of safe and efficacious nanocarriers is crucial to improving the bench-to-bedside success.
Solution: ProFECT(mRNA) Plus
Liposome mRNA Encapsulation (LSE) is a liposome assembly technique to enhance mRNA cargo loading into PEGylated neutral liposomes for improved biocompatibility and pharmacokinetics. This results in liposomal nanocarriers with efficient nucleic acid encapsulation and enhanced cellular association, while minimizing the cytotoxic effects associated with cationic-mediated transfection. LSE is a quick, easy, reproducible, scalable method for nanoparticle-mediated applied transfection.
Liposome mRNA Encapsulation (LSE) Kit
Description: Liposome mRNA Encapsulation (LSE) is a liposome assembly technique to enhance mRNA cargo loading into PEGylated neutral liposomes for improved biocompatibility and pharmacokinetics. This results in liposomal nanocarriers with efficient nucleic acid encapsulation and enhanced cellular association, while minimizing the cytotoxic effects associated with cationic-mediated transfection. LSE is a quick, easy, reproducible, scalable method for nanoparticle-mediated applied transfection.
LSE Kit Components:
- Composite lipid film (1mg per rxn)
- Lipid-based nucleic acid condenser (1 vial per rxn)
- Injection buffer (EtOH based buffer, 5ml per 10 rxn)
- Condensing buffer (Tris-HCl based buffer, 10ml per 10 rxn)
Not included in LSE kit:
- mRNA sequence for desired gene target
- dialysis cassette/tubing/etc. for liposome purification – OPTIONAL
- a quantitative measure for mRNA load (i.e. RiboGreen or other)- OPTIONAL
Storage Condition: 4°C for 6 months
Highly Translational: Comprised of natural lipid components
- Improved cellular association
- Low Toxicity: Eradicates toxicity due to charged liposomes
- Easy to Use Protocol: One-step assembly
- Enhanced encapsulation efficiency of the mRNA load
- Reduced nucleic acid leakage
Gene therapy has the potential to attenuate a variety of diseases by altering the molecular pathways leading to pathogenesis. However, the issue of delivery remains a primary hurdle in the successful translation of gene therapeutics. Cationic liposomes are beneficial experimental gene vectors due to electrostatic-mediated packaging of nucleic acids. However, the potential for clinical translation is limited in that cationic liposomes are associated with with reduced biocompatibility and poor pharmacokinetics. PEGylated neutral liposomes (PLPs), comprised of naturally occurring neutral lipids and surface grafted polyethylene glycol (PEG), are considerably more advantageous in terms of biocompatibility and increased drug stability. However, the addition of the PEG layer reduces the cell-associative properties of PLPs. Cell-penetrating peptides can be grafted onto the surface of liposomes to enhance cell delivery in order to overcome this so-called “PEG Dilemma”, but assembly must be controlled to prevent CPP-induced drug leakage.