Cationic Liposomes and Lipid Nanoparticles
Cationic liposomes are structures that are made of positively charged lipids and are vastly utilized in gene therapy due to their promising interactions with negatively charged cell membranes, RNA, and DNA.
|DOTAP Liposomes (1,3)|
|DOTAP-Chol Liposomes (2,3)|
|DOTAP-DOPC Liposomes (3)|
|DOTAP-DOPE Liposomes (2)|
|DOTAP-Chol-DOPE Liposomes (4)|
1) Porteous, D. J., et al. "Evidence for safety and efficacy of DOTAP cationic liposome mediated CFTR gene transfer to the nasal epithelium of patients with cystic fibrosis." Gene therapy 4.3 (1997): 210-218.
2) Li, S. M. A. S. L., et al. "Characterization of cationic lipid-protamine–DNA (LPD) complexes for intravenous gene delivery." Gene therapy 5.7 (1998): 930-937.
3) Templeton, Nancy Smyth, et al. "Improved DNA: liposome complexes for increased systemic delivery and gene expression." Nature biotechnology 15.7 (1997): 647-652.
4) Zhang, Ye, and Thomas J. Anchordoquy. "The role of lipid charge density in the serum stability of cationic lipid/DNA complexes." Biochimica et Biophysica Acta (BBA)-Biomembranes 1663.1-2 (2004): 143-157.