NanoSizer MINI

Frequently Asked Questions

NanoSizer MINI Frequently Asked Questions

What do I need to get started with extrusion?

Extrusion is easy with NanoSizerTM extruders. All you need is a Liposome solution, two syringes, and a NanoSizerTM extruder. The fully assembled extruders are available in different quantities. Heat blocks are also available for extrusion of high transition temperature lipids.

Do NanoSizer extruders include the membrane? What kind?

Each NanoSizerTM contains a track-etched polycarbonate membrane.

What kind of syringes are suitable for extrusion with NanoSizer?

NanoSizer extruders can be used with the needle-tipped Hamilton glass syringes provided with other common liposome mini-extruders. Plus, NanoSizer extruders accommodate fully assembled Luer-lock syringes: this means no cleaning of the syringe and no chance for leakage due to worn plunger assemblies. Both glass and single-use syringe are available here.

What kind of needles are required for extrusion with NanoSizer?

The needles provided with syringes are 22 Gauge and are designed with the right length for use with NanoSizer extruders. Replacement needles for glass and single-use syringes can be found here.

Can NanoSizer be heated? 

Yes, NanoSizerTM can be heated up to at least 100C using our heating block designed for a hot plate.

Do I need a heat block?

You will need to heat your solution above the transition temperature of the lipid-mixture (i.e. 41C for DPPC). A specially designed heat block that uses NanoSizer extruders is available here, and NanoSizerTM extruders are also compatible with other common heat blocks.

Is NanoSizer suitable for extrusion of high volume liposome solutions?

Yes, we have options for large volume extrusion needs. Please contact us at info@ttscientific.com

Will I get the same size distribution that I get with other extruders?

Yes, we have tested and compared the size distribution of liposomes created with NanoSizerTM and another common extruder via Dynamic Ligh Scattering (DLS). Results show equivalent size distribution via NanoSizerTM extrusion.

Do you have a liposome preparation protocol by extrusion?

Can the NanoSizer Cartridges be reused? 

The answer is yes. Each NanoSizer cartridge is able to extrude at least 20mL with NanoSizer MINI and at least 1L with a NanoSizer Plus. However, to avoid any chance of contamination we recommend using a new cartridge for any new experiment or new formulations and composition.

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