COVID-19 mRNA vaccines proved the potential of lipid nanoparticles as a robust and effective delivery systems. There is a lot of room to grow to make these nanoparticle more effective for other vaccines, and therapeutics. COVID vaccines have certainly expedited the use of these nanoparticles in wide range of gene therapies, cell therapies, specially for cancer and infectious diseases, vaccines and therapeutics. T&T Scientific is offering all inclusive formulation and manufacturing platform to help pharmaceutical companies with formulation and manufacturing of these vaccines, and therapeutics from preclinical stage to clinical phases and commercial manufacturing. We have a team who specializes in this area, and have initiated and executed over 20 RNA LNP formulation and manufacturing in preclinical, clinical phases, and commercial.
Ionizable Lipid Nanoparticles: Ionizable lipid nanoparticles are composed of three different segments: 1) the amine head group, 2) the linker section, and 3) the hydrophobic tails. These type of lipid nanoparticles are widely used for siRNA delivery with lipids such as DLinDMA and DLin–KC2–DMA. The mixing of cationic and anionic lipids in these nanoparticles help with the transfection in the way that the more stable lamellar phase translates to the less stable hexagonal phase, therefore aiding fusion of the liposomal and endosomal membranes (1-4). Solvent or ethanol injection is the most common technique to make these therapeutics and vaccines, as these techniques are low cost, fast, and provide desired size, size distribution polydispersity index (PDI), and encapsulation efficiency. We offer these services at our CDMO and has commercial equipment that can be installed and qualified at your site (5, 6).
Cationic Lipid Nanoparticles: Cationic lipid nanoparticles are also key components of lipid nanoparticles (LNP) delivery vehicles, which serve as a platform for delivery of various RNA, and therapeutics, and vaccine agents. Cationic lipid nanoparticles are increasingly regarded as one of the most promising mRNA delivery systems because of their good biocompatibility and easy large-scale production. Cationic lipid nanoparticle protect mRNA from degradation by nucleases and deliver the therapeutics and vaccines to cells by electrostatic adsorption of the charged lipids and fusion with the human cell membrane (3, 4). Solvent or ethanol injection is the most common technique to make these therapeutics and vaccines, as these techniques are low cost, fast, and provide desired size, size distribution polydispersity index (PDI), and encapsulation efficiency. We offer these services at our CDMO and has commercial equipment that can be installed and qualified at your site (5, 6).
Calcium-Phosphate Lipid Nanoparticles (CaP):These lipid nanoparticles are made of inner leaflet of a cationic lipid to encapsulate negatively charged polymers or nucleic acids. In cases neutral lipid is located on the outer leaflet to reduce non-specific cellular uptake and toxicity. The final nanoparticle is generated by adding free lipids to the cores. The formation of asymmetric bilayer happens as the organic solvent is removed from the mixture and exposed to an aqueous solution (7, 8). Solvent or ethanol injection is the most common technique to make these therapeutics and vaccines, as these techniques are low cost, fast, and provide desired size, size distribution polydispersity index (PDI), and encapsulation efficiency. We offer these services at our CDMO and has commercial equipment that can be installed and qualified at your site (5, 6).
Lipoplexes: Lipoplexes are another form of lipid delivery system, they are also known as cationic liposomes, are nonviral lipid nanoparticle carriers mainly used to encapsulate and deliver DNAs targetedly. Because of their positive surface charge, they complex well with negatively charged DNA. Lipoplexes in general made of of three components: a synthetic cationic lipid, a synthetic neutral lipid, and a plasmid DNA (10-15).